Menú superior

Home › Mass Spectrometry in Protein Analysis and Characterization

Mass Spectrometry in Protein Analysis and Characterization

EMBO World Practical Course
March 16th – 26th 2010
Institut Pasteur Montevideo
Montevideo, Uruguay

European Molecular Biology Organization

Intended (but not restricted) to PhD students and post-PhD fellows, young people involved in teaching and research at Universities or at public research institutes.

Organizers

  • Rosario Durán, PhD (Unit of Analytical Biochemistry and Proteomics-IP Montevideo and Instituto de Investigaciones Biológicas "Clemente Estable").
  • Carlos Batthyány, PhD (Unit of Analytical Biochemistry and Proteomics-IP Montevideo).
  • Carlos Cerveñansky, PhD (Unit of Analytical Biochemistry and Proteomics-IP Montevideo and Instituto de Investigaciones Biológicas "Clemente Estable").

Lecturers

  • Dr. Carlos Batthyány (IP Montevideo, Uruguay)
  • Dr. Juan José Cazzulo Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Buenos Aires)
  • Dr. Juan José Calvete (Structural Proteinomics Unit, Instituto de Biomedicina de Valencia, Spain)
  • Dr. Carlos Cerveñansky (IP Montevideo and IIBCE, Uruguay)
  • Dr Rosario Durán (IP Montevideo and IIBCE, Uruguay)
  • Dr. Gustavo Goldman (Faculda e de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil)
  • Dr. Luis Javier González (Centro de Ingeniería Genética y Biotecnología (CIGB) La Habana, Cuba)
  • Dr. Ulf Hellman (Protein Structure Group, Ludwing Institute for Cancer Research, Uppsala Branch, Sweden)
  • Dr. Silvia Moreno (Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM) FCEyN Universidad de Buenos Aires, Argentina)
  • Dr. Francisco Schopfer (Department of Pharmacology & Chemical Biology, University of Pittsburgh, PA, USA)
  • Dr. José Souza (Departamento de Bioquímica, Facultad de Medicina, UdelaR, Uruguay)
  • Dr. Gerald Spaeth (Département de Parasitologie et Mycologie, Institut Pasteur, Paris, France)
  • Dr. Mirtha Biscoglio (Laboratorio Nacional de Investigación y Servicios en Péptidos y Proteínas (LANAIS-PRO), Buenos Aires, Argentina)
  • Dr. Susana Linskens (Laboratorio Nacional de Investigación y Servicios en Péptidos y Proteínas (LANAIS-PRO), Buenos Aires, Argentina)
  • PhD. Maria Prieto Conaway (Thermo Fisher Scientific, USA)
  • M.Sc. Luis Servin Vega (ABSciex, Mexico)

Practical Instructors

  • Madelon Portela (IP Montevideo/Facultad de Ciencias, Uruguay)
  • Analia Lima (IP Montevideo, Uruguay)
  • Magdalena Gil (IIBCE, Uruguay)
  • Florencia Festari (IP Montevideo, Uruguay)

How to apply

Aplicants must send the following documents in pdf to: mass.spectrometry@pasteur.edu.uy:

  1. Brief CV (max. 2 pages)
  2. Letter of intent with a brief description of research interests and the benefits to attend to this course
  3. Letter of support signed by the advisor

A number of travel grants for Latinamerican fellows would be available.

Deadline: December 15th, 2009

Scholarship

Inscription fees are fully covered for all accepted students. Applicants from abroad with no financial support, are welcome to apply for a lodging and per diem expenses scholarship. This should be inlcuded as a separate document at the time of application. A limited number of traveling aids will be available: arguments backing this request should be duly explained, to be evaluated on a case by case basis.

Preliminary program

The lecture program will include the following items:

  1. MS basic definitions: ionization methods, mass analyzers, instrumentation (MALDI-TOF, ESI- quadrupole and ESI- ion traps). Tandem instruments. General applications in the field of biological/biomedical research.
  2. Introduction to protein chemistry and biochemistry. MS in protein research: overview in protein analysis, structure and functional characterization and quantitation. Proteomics.
  3. Important performance factors: sensitivity, resolution, mass accuracy. Tandem MS/MS. Fragmentation techniques (CID, PSD, ETD).
  4. MALDI TOF sample preparation. Relevant association of classical biochemical methods to sample preparation: HPLC, affinity chromatography, chemical derivatization, 1D and 2D electrophoresis. DIGE technology.
  5. Molecular mass determination of proteins and peptides in solution.
  6. Protein identification by PMF in gel spot samples (proteolytic enzymes, data acquisition and analysis, protein data bank searching). Shot-gun proteomics. LC-MS methods.
  7. PTM identification and localization by MS and MS/MS methods. Examples including phosphorylation, glycosylation, nitration, oxidations. Determination of SH and SS bridges.
  8. Introduction to quantitative proteomic methods. Determination of protein expression levels. Other applications in biomedicine: biomarker discovery, microorganism identification, quality control of recombinant proteins, etc.

The practical course will include the following items:

  1. Examples of basic procedures. Molecular mass determination of a protein and in a simple peptide mixture in solution. Analysis of the information provided by a mass spectrum. Evaluation of resolution, sensitivity, data quality, effects of salts, detergents, etc.
  2. Sample preparation for MALDI TOF measurements: peptide analysis by RP-HPLC, sample plate seeding, types of matrix, matrix addition.
  3. Sample preparation: 2D gel and spot processing, protein identification by PMF and protein data bank search. Searching programs. Contamination problems from the environment dust.
  4. Identification of phosphorylation sites: affinity enrichment methods, MS/MS.
  5. Identification of o-glycosylation sites; time course analysis of a glycosylation reaction.
  6. "Shot-gun" proteomics (ESI-ion trap instrument is expected to be available at the time of the course).
  7. Optional: analysis of samples provided by the students.

More information: mass.spectrometry@pasteur.edu.uy

This course is supported by: EMBO - AMSUD/Pasteur - IP MONTEVIDEO (URUGUAY INNOVA)
Sponsored by: PEDECIBA Biología - PEDECIBA Química