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Equipment

 

Cell Culture Facility

UNDER CONSTRUCTION

 

 

 

 

Flow Cytometry

 

MoFlow high speed cell sorter (Beckman Coulter)

 

This instrument has the capability to:

  • Sort a total event rate of over 30,000 per second 
  • Analyze a total event rate of over 50,000 per 
  • Up to 4 populations can be sorted simultaneously (4-way sorting) 
  • Cell/well cloning directly into tissue culture trays with up to 96 wells/tray

 

It is equipped with:

              

 


CyAn Flow Cytometer (Beckman Coulter)

CyAn ADP can measure the following parameters: 
  • Size of a cell: Forward light Scatter (FSC) expresses the. 
  • Granularity of a cell: Side light Scatter (SSC) expresses the. 
  • Fluorescent parameters: 

                  

  • Fluorescence resolution: coefficient of variation (CV) is <3% full peak for PE & FITC SpectrAlign beads. 
  • Flow rates: Up to 150 µl/min 
  • Processing speed: up to 70,000 events per second. 
  • Analysis capability: up to 100,000,000 event data files

 

Data analysis

The cell biology Unit provides the following flow cytometry data analysis software:

  • SUMMIT 4.3
  • FlowJo 7.6.4
  • FCSExpress 4 Flow research edition

Technical assistance is offer in sample preparation, reagent purchase, experimental design and experimental data analysis.

Trainings and education is available for all the new users. 

We are also open to establish collaborations and have an active involvement in your research providing intellectual and practical assistance.

For more information and bookings you can contact us: citometria@pasteur.edu.uy

 

 

 

Microscopy Facility

 

 

Epifluorescence Microscope Olympus IX81

  • Fixed or live samples 
  • 2D imaging, timelapse, photoactivation 
  • Camera: Hamamatsu Orca ER
  • Filter Wheel: U-MNUA2: Excitation 360-370nm/Emission 420-460nm U-MNIBA3:Excitation 470-495nm/Emission 510-550nm, U-MWIG3: Excitation 530-550nm/Emission 570nm 
  • Objectives: 10X NA 0.30, 20X NA 0.50, 40X NA 0.65, 40x LWD NA 0.6, 100X NA 1.40 oil
  • Image acquisition and processing is made through Image Pro v6.1.                                                                                          

 

Spectral Confocal Microscope Leica TCS SP5 

  • Inverted Microscope Leica DMI6000CS  
  • Fixed or live samples with temperature and CO2 control (Ludin cube)
  • Two spectral tunable detectors 
  • Fast resonant scanner 
  • 3D z-stacks, timelapse, FRAP/FLIP/Photoactivation, FRET 
  • Filter Wheel: A: Excitation 340-380nm/Emission 425, I3: Excitation 450-490nm/Emission 515nm, N2.1: Excitation 515-560nm/Emission 590nm
  • Objectives: 20x  NA 0.70 water / glycerol /oil   63X NA 1.42 oil         
  • Laser lines available: Argon 488nm: 458/476/488/496/514, HeNe 543nnm, HeNe 594nm, HeNe 633nm                          
  • Image acquisition and processing is made through LASAF v2.2.0.

 

 

 

Technical Assistance

The microscopy facility offers assistance in sample preparation, reagent purchases, image acquisition, troubleshooting and image processing and analysis. 

 Trainning and education is available for all the new users. 

We are also open to establish collaborations and have an active involvement in your research providing intellectual and practical assistance.

For more information and bookings you can contact us: microscopia@pasteur.edu.uy