Recombinant Proteins Unit
Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural protein sources rarely meet the requirements for quantity, ease of isolation or price and hence recombinant technology is often the method of choice. Recombinant cell factories are constantly employed for the production of protein preparations bound for downstream purification and processing.
The purpose of the Recombinant Prtotein Unit (RPF) at the Pasteur Institute of Montevideo (IPMON) is to apply state-of-the-art technologies to produce functional recombinant proteins expressed in prokaryotic and eukaryotic systems.
The goals of this facility core are:
- To provide the means of generating alternative gene cloning constructs when required.
- To optimize and automate the expression and purification of target proteins.
- To generate solubilization strategies in the case of insoluble proteins.
In every case, convenient scaling-up strategies will be available in order to cope with the amounts of recombinant material required for protein structure/functional studies.
Head
- Pablo Oppezzo, PhD
Members
- Cecilia Abreu (PhD student-staff Research Assistant)
- Florencia Palacios (PhD student-staff Research Assistant)
- Agustín Correa (MSc student-staff Research Assistant)
- Martin Puñales (Student) Pilar Moreno (Ph.D)
Past members
- Paola Scavone
- Pablo Morande